Embryonic Cells Redistribute SUMO1 upon Forced SUMO1 Overexpression
نویسندگان
چکیده
منابع مشابه
Specification of SUMO1- and SUMO2-interacting motifs.
SUMO proteins are ubiquitin-related modifiers implicated in the regulation of gene transcription, cell cycle, DNA repair, and protein localization. The molecular mechanisms by which the sumoylation of target proteins regulates diverse cellular functions remain poorly understood. Here we report isolation and characterization of SUMO1- and SUMO2-binding motifs. Using yeast two-hybrid system, bioi...
متن کاملCommentary: Analysis of SUMO1-conjugation at synapses
Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, IPMC, Université Côte d’Azur, Nice, France, 3 Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland, 4 Taub Institute and Department of Pathology and Cell Biology, Columbia University, New York, NY, United States, 5 Department of Applied Sciences, University of the West of England, B...
متن کاملAnalysis of SUMO1-conjugation at synapses
SUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and immunostaining analyses of synaptic SUMO1-conjugation. By using a knock-in repor...
متن کاملComparison of the SUMO1 and ubiquitin conjugation pathways during the inhibition of proteasome activity with evidence of SUMO1 recycling.
To investigate potential interplay between the SUMO1 (small ubiquitin-related modifier-1) and ubiquitin pathways of post-translational protein modification, we examined aspects of their localization and conjugation status during proteasome inhibition. Our results indicate that these pathways converge upon the discrete sub-nuclear domains known as PML (promyelocytic leukaemia protein) NBs (nucle...
متن کاملIdentification of endogenous SUMO1 accepter sites by mass spectrometry.
Posttranslational modification (PTM) by the covalent conjugation of small ubiquitin-like modifier (SUMO) plays an important role in many biological processes, such as cell cycle progression, transcriptional regulation, subcellular transport, and other processes. An in-depth understanding of the function of SUMOylation requires the discovery of SUMO accepter sites. However, identification of end...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: mBio
سال: 2019
ISSN: 2161-2129,2150-7511
DOI: 10.1128/mbio.01856-19